ABSTRACT
Blood and lymphatic vessel proliferation is essential for tumor growth and progression. Most colorectal carcinomas develop from adenomas (adenoma-carcinoma sequence) in a process due to accumulation of molecular genetic alterations. About 5% of adenomatous polyps are expected to become malignant, but data on the differential angiogenic patterns of these lesions in patients with and without concomitant cancer are missing. The aim of the present study is to compare the angiogenic and lymphatic patterns of adenomatous polyps from patients with and without sporadic cancer. Thirty adenomatous polyps (15 from patients with another principal malignant lesion, and 15 from patients without cancer) were submitted to immunohistochemical staining for CD105 (marker for neoangiogenesis) and D2-40 (marker for lymphatic endothelium). Microvessel density and total vascular area were determined by computer image analysis to quantify the immunostained and total areas, and to assess the number of microvessels. Adenomas from patients with carcinoma showed significantly higher values of total vascular area determined by immunostaining for CD105 (cutoff value = 4386 µm²; P = 0.019) and of lymphatic microvessel density determined by immunostaining with D2-40 (cutoff value = 11.5; P = 0.041) when compared with those from patients without cancer. The present data indicate a significant increase in blood microvascular area and in lymphatic microvascular counts in adenomas removed from patients with cancer.
Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Adenomatous Polyps/pathology , Colorectal Neoplasms/pathology , Lymphangiogenesis/physiology , Neovascularization, Pathologic/pathology , Adenomatous Polyps/blood supply , Adenomatous Polyps/chemistry , Antibodies, Monoclonal/analysis , Antigens, CD/analysis , Biomarkers/analysis , Colorectal Neoplasms/blood supply , Colorectal Neoplasms/chemistry , Immunohistochemistry , Lymphatic Vessels/chemistry , Lymphatic Vessels/pathology , Microcirculation , Retrospective Studies , Receptors, Cell Surface/analysisABSTRACT
CD95 (Fas/APO-1)-mediated apoptosis plays an important role in immunological regulation and is related to the pathogenesis of autoimmune diseases. Immunoexpression of CD95 has been reported to frequently occur in low grade non-Hodgkin lymphomas, especially of post-germinal center histogenesis, among which those originating in mucosa-associated lymphoid tissue (MALT lymphomas). However, there is no report comparing in situ immunoexpression of this marker in lymphomas and the hyperplastic lymphoid reaction (chronic gastritis) related to Helicobacter pylori infection. The purpose of the present research was to compare the intensity of lymphoid CD95 immunoexpression in 15 cases of H. pylori-related chronic gastritis and 15 gastric MALT lymphomas. CD95 (anti-CD95) was detected by an immunoperoxidase technique in paraffin sections using the catalyzed amplification system. Graduation of reaction intensity (percentage of CD95-positive cells) was semiquantitative, from 1+ to 4+. Nine cases of chronic gastritis were 4+, five 2+ and one 1+. Three lymphomas were 4+, three 3+, four 2+, four 1+, and one was negative. Although 14 of 15 lymphomas were positive for CD95, the intensity of the reaction was significantly weaker compared to that obtained with gastric tissue for patients with gastritis (P = 0.03). The difference in CD95 immunoexpression does not seem to be useful as an isolated criterion in the differential diagnosis between chronic gastritis and MALT lymphomas since there was overlapping of immunostaining patterns. However, it suggests the possibility of a pathogenetic role of this apoptosis-regulating protein in MALT lymphomas.
Subject(s)
Humans , fas Receptor , Apoptosis , Gastritis , Lymphoma, B-Cell, Marginal Zone , Stomach Neoplasms , Biomarkers, Tumor , Chronic Disease , Diagnosis, Differential , Helicobacter Infections , Helicobacter pylori , Immunoenzyme Techniques , ImmunohistochemistryABSTRACT
Prevalência da gastrite crônica e da infecçäo da mucosa gástrica por Helicobacter pylori em pacientes com dispepsia näo ulcerosa e com úlcera duodenal. Tipo de estudo, local, pacientes: Foram estudados, prospectivamente, 48 pacientes consecutivos com dispepsia näo ulcerosa (DNU) do tipo dismotilidade e 13 pacientes consecutivos com úlcera duodenal (UD) em atividade, selecionados no ambulatório de Gastrenterologia do Hospital das Clínicas da Universidade Estadual de Campinas (Unicamp). Intervençöes: Em cada um dos pacientes, foram realizadas oito biópsias endoscópicas (quatro do corpo e quatro do antro gástrico), para identificaçäo do H. pylori, utilizando-se três testes: urease, gram e exaqme histopatológico. Medidas e resultados: Nos 48 pacientes com DNU, o teste de urease foi postivo em 89,6% no antro e 81,2% no corpo; o gram foi positivo em 81,2% no antro e em 77,1% no corpo; e o H. pylori foi identificado no exame histopatológico em 79,2% no antro e em 70,8% no corpo gástrico. Na mucosa do antro gástrico de todos os 48 pacientes com DNU, pelo menos um dos três testes empregados foi positivo. Em todos os 13 pacientes com UD, os três testes foram positivos no antro gástrico, todos com gastrite crônica do antro no exame histopatológico. Gastrite crônica do corpo gástrico, com presença do HY. pylori no exame histopatológico, foi encontrada em 10 dos 13 pacientes. O teste de urease foi positivo na mucosa do corpo gástrico em todos os casos de UD. Conclusöes: Os autores acreditam que a alta prevalência dos testes empregados para identificaçäo fo H. pylori nos pacientes com DNU possa ser explicada pelo grupo selecionado de pacientes pertencentes a uma classe socioeconômica menos favorecida. A presença do H. pyloru na mucosa gástrica de todos os pacientes com UD está de acordo com algums trabalhos já publicados